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Catalog Number Size Price
LS-C662611-10 10 µg $318 
LS-C662611-100 100 µg $470 
DNMT / DNMT1 Antibody - IHC analysis of Dnmt1 using anti-Dnmt1 antibody. Dnmt1 was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Dnmt1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DNMT / DNMT1 Antibody - Immunohistochemistry - Anti-Dnmt1 Picoband antibody
DNMT / DNMT1 Antibody - Immunohistochemistry - Anti-Dnmt1 Picoband antibody
DNMT / DNMT1 Antibody - Immunohistochemistry - Anti-Dnmt1 Picoband antibody
DNMT / DNMT1 Antibody - IF analysis of Dnmt1 using anti-Dnmt1 antibody Dnmt1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Dnmt1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
DNMT / DNMT1 Antibody - Western blot - Anti-Dnmt1 Picoband antibody
DNMT / DNMT1 Antibody - Flow Cytometry analysis of A431 cells using anti-Dnmt1 antibody. Overlay histogram showing A431 cells stained with anti-Dnmt1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Dnmt1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
DNMT / DNMT1 Antibody - IHC analysis of Dnmt1 using anti-Dnmt1 antibody. Dnmt1 was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Dnmt1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DNMT / DNMT1 Antibody - Immunohistochemistry - Anti-Dnmt1 Picoband antibody
DNMT / DNMT1 Antibody - Immunohistochemistry - Anti-Dnmt1 Picoband antibody
DNMT / DNMT1 Antibody - Immunohistochemistry - Anti-Dnmt1 Picoband antibody
DNMT / DNMT1 Antibody - IF analysis of Dnmt1 using anti-Dnmt1 antibody Dnmt1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Dnmt1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
DNMT / DNMT1 Antibody - Western blot - Anti-Dnmt1 Picoband antibody
DNMT / DNMT1 Antibody - Flow Cytometry analysis of A431 cells using anti-Dnmt1 antibody. Overlay histogram showing A431 cells stained with anti-Dnmt1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Dnmt1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human DNMT / DNMT1 Antibody (IHC, WB) LS‑C662611

Polyclonal Rabbit anti‑Human DNMT / DNMT1 Antibody (IHC, WB) LS‑C662611

Antibody:
DNMT / DNMT1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662611-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
DNMT / DNMT1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DNMT1 antibody LS-C662611 is an unconjugated rabbit polyclonal antibody to human DNMT1 (DNMT). Validated for ELISA, IHC and WB.
Target
Human DNMT / DNMT1
Synonyms
DNMT1 | AIM | CXXC finger protein 9 | CXXC9 | DNA methyltransferase 1 | DNMT | DNA methyltransferase HsaI | DNA MTase HsaI | HSN1E | M.HsaI | MCMT
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived human Dnmt1 recombinant protein (Position: D22-N126).
Specificity
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DNMT / DNMT1
P26358 NM_001379 NP_001370.1

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Requested From: United States
Date Requested: 11/22/2024