Human EGF

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Monoclonal

R001

Unconjugated

Protein A purified

Preservative Free

Recombinant Human EGF / Epidermal Growth Factor protein

Human EGF. Has cross-reactivity with Canine EGF and Mouse EGF in ELISA assay

• ELISA (1.2 µg/ml)
• Blocking (5 µg/ml)
• Neutralization (2 ng/ml)

Block: In a functional ELISA, 1. 2 µg/ml of this antibody will block >80% of the binding of 5 µg/ml of biotinylated Human EGF to immobilized Recombinant Human EGFR coated at 10 µg/ml (100 µL/well) in the plate. Neutralization: The neutralization activity of EGF Neutralizing Antibody is Measured by its ability to neutralize EGF induced proliferation in the Balb/c 3T3 mouse embryonic fibroblast cell line. The Neutralization titer (IC50) is typically 2. 5-7. 5 ug/ml in the presence of 2 ng/mL Recombinant Human EGF. Please Note: Optimal concentrations/dilutions should be determined by the end user.

0.2 µm filtered histidine and arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH 6.0

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01133 NCBI: NM_001963 NP_001954.2