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Antibodies > EIF2AK3 / PERK > LS-C353177
 
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Catalog Number Size Price
LS-C353177-100 100 µl (1 mg/ml) $299 
EIF2AK3 / PERK Antibody - Immunohistochemical analysis of PERK staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
EIF2AK3 / PERK Antibody - Immunofluorescent analysis of PERK staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
EIF2AK3 / PERK Antibody - Western blot analysis of PERK expression in HeLa (A) whole cell lysates.
EIF2AK3 / PERK Antibody - Immunoprecipitation of PERK from 0.5mg HeLa whole cell extract lysate using 5ug of Anti-PERK Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-PERK Antibody.
EIF2AK3 / PERK Antibody - Immunohistochemical analysis of PERK staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
EIF2AK3 / PERK Antibody - Immunofluorescent analysis of PERK staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
EIF2AK3 / PERK Antibody - Western blot analysis of PERK expression in HeLa (A) whole cell lysates.
EIF2AK3 / PERK Antibody - Immunoprecipitation of PERK from 0.5mg HeLa whole cell extract lysate using 5ug of Anti-PERK Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-PERK Antibody.
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Polyclonal Rabbit anti‑Human EIF2AK3 / PERK Antibody (N‑Terminus, IHC, IF, WB) LS‑C353177

Polyclonal Rabbit anti‑Human EIF2AK3 / PERK Antibody (N‑Terminus, IHC, IF, WB) LS‑C353177

Submit Review LSBio Guarantee
Antibody:
EIF2AK3 / PERK Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C353177-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
EIF2AK3 / PERK Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PERK antibody LS-C353177 is an unconjugated rabbit polyclonal antibody to human PERK (EIF2AK3) (N-Terminus). Validated for ICC, IF, IHC, IP and WB.
Target
Human EIF2AK3 / PERK
Synonyms
EIF2AK3 | HRI | HsPEK | Pancreatic eIF2-alpha kinase | PEK | PERK | PKR-like ER kinase | WRS
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human PERK.
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of PERK protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EIF2AK3 / PERK
Q9NZJ5 NM_004836 NP_004827.4

Publications (0)

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Featured Products

Product
EIF2AK3 / PERK Antibody (phospho‑Thr980) LS‑C38147
Species: Mouse, Rat
Applications: Western blot, ELISA
EIF2AK3 / PERK Antibody - Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
EIF2AK3 / PERK Antibody (aa148‑175) LS‑C101136
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
EIF2AK3 / PERK Antibody - Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PEK/PERK Antibody. The picture on the right is blocked with the synthesized peptide.
EIF2AK3 / PERK Antibody (aa947‑996) LS‑C118320
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
EIF2AK3 / PERK Antibody - Western blot using EIF2AK3 monoclonal antibody against HEK293 (1) and EIF2AK3(AA: 929-1116)-hIgGFc transfected HEK293 (2) cell lysate.
EIF2AK3 / PERK Antibody (clone 5G5) LS‑C169265
Species: Human
Applications: Western blot, ELISA
EIF2AK3 / PERK Antibody - Immunohistochemical analysis of PERK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
EIF2AK3 / PERK Antibody (N‑Terminus) LS‑C413097
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
EIF2AK3 / PERK ELISA Kit
Human EIF2AK3 / PERK (Sandwich ELISA) ELISA Kit - LS-F11339
Reactivity: Human
Range: 0.312-20 ng/ml
See all EIF2AK3 / PERK Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/1/2024
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