Target
Human EIF4EBP1 / 4EBP1
Synonyms
EIF4EBP1 | 4E-BP1 | 4EBP1 | BP-1 | PHAS-I | EIF4E-binding protein 1
Reactivity
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1(KLH-coupled).
Specificity
Recognizes endogenous levels of human 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at analagous sites. Species cross-reactivity: mouse, rat, monkey.
Applications
- Western blot (1:1000)
- Flow Cytometry (1:400)
Usage
Suitable for use in Western Blot and Flow Cytometry. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%. Tween-20 at 4°C with gentle shaking, overnight. Flow Cytometry: 1:400. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee