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| Catalog Number | Size | Price |
|---|---|---|
| LS-C204514-100 | 100 µl | $376 |
1 of 2
2 of 2
Monoclonal Mouse anti‑Human ENO1 / Alpha Enolase Antibody (clone 253, IF, WB) LS‑C204514
Monoclonal Mouse anti‑Human ENO1 / Alpha Enolase Antibody (clone 253, IF, WB) LS‑C204514
Antibody:
ENO1 / Alpha Enolase Mouse anti-Human Monoclonal (253) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Xenopus
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
ENO1 / Alpha Enolase Mouse anti-Human Monoclonal (253) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Xenopus
Format:
Unconjugated, Unmodified
Specifications
Description
Alpha Enolase antibody LS-C204514 is an unconjugated mouse monoclonal antibody to Alpha Enolase (ENO1) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF and WB.
Target
Human ENO1 / Alpha Enolase
Synonyms
ENO1 | Alpha Enolase | Alpha enolase like 1 | ENO1L1 | Enolase 1 | MBP-1 | MBPB1 | MPB1 | MYC promoter-binding protein 1 | NNE | Phosphopyruvate hydratase | Alpha-enolase | Tau-crystallin | MBP1 | MPB-1 | C-myc promoter-binding protein | Enolase 1, (alpha) | Enolase-alpha | Non-neural enolase | Plasminogen-binding protein | PPH
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Xenopus
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
253
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
N-Terminus 12 amino acids of bovine enolase 1 which was synthesized on a 8-amine lysine core using the multiple antigen presentation method. This produces a dendrimer presenting 8 peptides to the immune system.
Specificity
Clone 253 is known to react with enolase 1 from human, bovine, mouse, rat and Xenopus. The antibody was tested for binding to expressed bovine enolase 1, 2 and 3 and shown to be specific for only enolase 1.
Applications
- ICC (1:2000 - 1:5000)
- Immunofluorescence (1:2000 - 1:5000)
- Western blot (1:5000 - 1:10000)
Usage
Try at dilutions of 1:2000-1:5000 for immunofluorescence. For western blots try at 1:5000-1:10000. The NNE protein runs at about ~47 kDa on SDS-PAGE gels.
Presentation
PBS, 10 mM Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ENO1 / Alpha Enolase
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
