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Catalog Number	Size	Price
LS-C287259-10	10 µl	$295
LS-C287259-100	100 µl (1 mg/ml)	$514

EP300 / p300 Antibody - Detection of human p300 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-p300 used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

EP300 / p300 Antibody - Detection of human p300 by western blot. Samples: Whole cell lysate from HeLa (15, 5 µg), HEK293T (15, 5 µg), and Jurkat (50 µg)cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p300 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

EP300 / p300 Antibody - Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

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Polyclonal Rabbit anti‑Human EP300 / p300 Antibody (aa950‑1000, IHC, WB) LS‑C287259

Name: Anti-EP300 / p300 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C287259-10
Price: 295.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

EP300 / p300 Rabbit anti-Human Polyclonal (aa950-1000) Antibody

Application:

IHC, IHC-P, WB, IP, ChIP-Seq

Reactivity:

Human

Format:

Unconjugated, Unmodified

$295

LS-C287259-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

EP300 / p300 Rabbit anti-Human Polyclonal (aa950-1000) Antibody

Application:

IHC, IHC-P, WB, IP, ChIP-Seq

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

P300 antibody LS-C287259 is an unconjugated rabbit polyclonal antibody to human p300 (EP300) (aa950-1000). Validated for ChIP-Seq, IHC, IP and WB.

Target

Human EP300 / p300

Synonyms

See All EP300 / p300 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purified

Modifications

Unmodified

Immunogen

A portion of GeneID: 2033 corresponding to amino acids between 950 and 1000 of UniprotID: Q09472

Epitope

aa950-1000

Specificity

Region between residues 950 and 1000 of human E1Aassociated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033).

Applications

IHC
IHC - Paraffin (1:1000 - 1:5000)
Western blot (1:2000 - 1:10000)
Immunoprecipitation (1 - 5 µg/mg lysate)
ChIP-sequencing

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ChIP-Seq: 10 µg

Manufacturer

Bethyl Laboratories, Inc.

Presentation

Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.

Storage

Store at 2-8°C for up to one year.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.