Human EPCAM

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

HEA125

Unconjugated

Purified

Unmodified

Viable HT-29 human colon carcinoma cell line

The antibody HEA125 is directed against human EpCAM. he monoclonal antibody HEA125 shows a broad reactivity pattern with most human epithelial cells and epithelium derived tumours. It doesn't react with epidermal keratinocytes, parietal cells, hepatocytes, thymus cortical epithelium, myoepithelia, and non-epithelial tissues. HEA125 reacts intensely with all carcinomas derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate and metastases thereof. Stratified squamous epithelia usually stain less intensely then adenocarcinoma. The antibody is suitable for differentiation between carcinoma and non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained as sarcoma, lymphoma, melanoma and neurogenic tumours do.

• IHC - Paraffin (1:100 - 1:500)
• IHC - Frozen (1:100 - 1:500)
• Immunofluorescence
• Flow Cytometry
• ELISA

Applications should be user optimized.

PBS, 0.09% Sodium Azide

Store at 4°C for short term storage. Aliquot and store at -20°C for long term storage. Avoid freeze/thaw cycles. Prior to use, thaw aliquot slowly in the dark at ambient temperature. Spin down and prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Store working dilutions at 4°C, and preferably use the same day. Remove slight precipitation with centrifugation.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16422 NCBI: NM_002354 NP_002345.2