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| Catalog Number | Size | Price |
|---|---|---|
| LS-C23274-50 | 50 µg (1 mg/ml) | $642 |
| LS-C23274-200 | 200 µg | $1,033 |
Monoclonal Mouse anti‑Human ER Alpha / Estrogen Receptor Antibody (clone C0542, aa582‑595, IHC, WB) LS‑C23274
Monoclonal Mouse anti‑Human ER Alpha / Estrogen Receptor Antibody (clone C0542, aa582‑595, IHC, WB) LS‑C23274
Antibody:
ER Alpha / Estrogen Receptor Mouse anti-Human Monoclonal (aa582-595) (C0542) Antibody
Application:
IHC, ICC, WB, IP, GS
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
ER Alpha / Estrogen Receptor Mouse anti-Human Monoclonal (aa582-595) (C0542) Antibody
Application:
IHC, ICC, WB, IP, GS
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Estrogen Receptor antibody LS-C23274 is an unconjugated mouse monoclonal antibody to human Estrogen Receptor (ER Alpha) (aa582-595). Validated for GS, ICC, IHC, IP and WB.
Target
Human ER Alpha / Estrogen Receptor
Synonyms
ESR1 | Era | ERalpha | Estrogen receptor | ER-alpha | Estrogen receptor 1 | NR3A1 | ER | ESR | ESRA | Estrogen receptor alpha
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
C0542
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A 14 residue C-Terminus synthetic peptide, (C)YYITGEAEGFPATV based on the human estrogen receptor (aa 582-595) alpha protein sequence with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon (100%); Gorilla, Monkey (93%); Bat, Bovine, Horse (86%).
Epitope
aa582-595
Specificity
Recognizes the human estrogen receptor at ~65kD and ~55kD believed to be the major proteolytic fragment of the estrogen receptor alpha protein. The antibody also recognizes native estrogen receptor in both the inactive 8S oligomeric state, when activated to 4S and whether unbound or bound to ligand. Species cross-reactivity: mouse, rat and hamster.
Applications
- IHC
- ICC
- Western blot
- Immunoprecipitation
- Gel shift
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Western Blot, Gel Mobility Shift Assay, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot (ECL): 2 ug/ml. Gel Mobility Shift Assay: 20 ug/reaction. Immunoprecipitation: 20 ug/100 ul beads. We recommend that protein A Sepharose be used as immunoabsorbant with a bridging rabbit anti-mouse IgG to ensure efficient binding of the antibody.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ER Alpha / Estrogen Receptor
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Customer Reviews (0)
Featured Products
Species:
Bovine, Human, Mouse
Applications:
IHC, IHC - Frozen, Western blot, Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, Western blot, Immunoprecipitation, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Reactivity:
Human, Mouse
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
