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Catalog Number	Size	Price
LS-C154240-100	100 µg (0.56 mg/ml)	$567

GDF15 Antibody - Anti-NAG-1 (D variant specific) Antibody - ELISA. In this sandwich ELISA, NAG-1 was captured from human serum using the following antibodies (see Related Products below): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 ug/mL. Control plates received PBS only (data not shown). After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C. After washing, HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 ul per well at 1 ug/mL. Following further incubation for 1 hr at 37°C, the plates were washed and TMBE was added as an HRP substrate for 30 min. The reaction was stopped by 1 M H2SO4 and values were measured at 450nm.

Polyclonal Rabbit anti‑Human GDF15 Antibody (IHC, WB) LS‑C154240

Name: Anti-GDF15 Antibody | Rabbit anti-Human Polyclonal ELISA,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C154240-100
Price: 567.0000 USD
Availability: InStock

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Antibody:

GDF15 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$567

LS-C154240-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

GDF15 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

GDF15 antibody LS-C154240 is an unconjugated rabbit polyclonal antibody to human GDF15. Validated for ELISA, IHC and WB.

Target

Human GDF15

Synonyms

GDF15 | MIC1 | MIC-1 | NAG-1 | NRG-1 | NSAID-regulated gene 1 protein | PLAB | NSAID-activated gene 1 protein | Placental TGF-beta | PTGFB | GDF-15 | PTGF-beta

See All GDF15 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purified

Modifications

Unmodified

Immunogen

This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.

Specificity

This antibody specifically reacts with a D variant sequence of human NAG-1 protein from human tissues. A BLAST analysis was used to suggest partial reactivity with NAG-1 from chimpanzee and macaque based on a 92% homology. Cross-reactivity with NAG-1 from other sources has not been determined.

Applications

IHC
Western blot (1:2000 - 1:10000)
ELISA (1:10000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

This affinity purified antibody is suitable for use in ELISA and western blotting assays. This reagent is particularly useful to differentiate polymorphic forms of NAG-1 protein present in human serum samples. This antibody is useful in dual antibody immunometric assays (EIA). Specific conditions for reactivity should be optimized by the end user.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About GDF15

Uniprot: Q99988 NCBI: NM_004864 NP_004855.2