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Catalog Number	Size	Price
LS-C358767-30	30 µl (1 mg/ml)	$255
LS-C358767-100	100 µl (1 mg/ml)	$299
LS-C358767-200	200 µl (1 mg/ml)	$351

H2AFX / H2AX Antibody - Immunohistochemical analysis of Histone H2A.X staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

H2AFX / H2AX Antibody - Immunofluorescent analysis of Histone H2A.X staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

H2AFX / H2AX Antibody - Western blot analysis of Histone H2A.X expression in HepG2 (A); mouse brain (B) whole cell lysates.

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Polyclonal Rabbit anti‑Human H2AFX / H2AX Antibody (C‑Terminus, IHC, IF, WB) LS‑C358767

Name: Anti-H2AFX / H2AX Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C358767-30
Price: 255.0000 USD
Availability: InStock

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Antibody:

H2AFX / H2AX Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$255

LS-C358767-30

$299

LS-C358767-100

$351

LS-C358767-200

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

H2AFX / H2AX Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

H2AX antibody LS-C358767 is an unconjugated rabbit polyclonal antibody to H2AX (H2AFX) (C-Terminus) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB.

Target

Human H2AFX / H2AX

Synonyms

See All H2AFX / H2AX Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human Histone H2A. X.

Epitope

C-Terminus

Specificity

Recognizes endogenous levels of Histone H2A. X protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence (1:100 - 1:500)
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About H2AFX / H2AX

Uniprot: P16104 NCBI: NM_002105 NP_002096.1