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Catalog Number Size Price
LS-C287018-10 10 µl $295 
LS-C287018-100 100 µl (1 mg/ml) $533 
H2AFX / H2AX Antibody - Detection of human gamma-H2AX by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- gamma-H2AX used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB.
H2AFX / H2AX Antibody - Detection of mouse gamma-H2AX by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- gamma-H2AX used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB.
H2AFX / H2AX Antibody - Detection of Human gamma-H2AX by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-gamma-H2AX ( Lot12) used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
H2AFX / H2AX Antibody - Detection of Human gamma-H2AX by Immunocytochemistry. Samples: Neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right). Antibody: Affinity purified rabbit anti-gamma-H2AX ( Lot12) used at a dilution of 1:5000 (0.2 ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight 594 conjugated used at a dilution of 1:100.
H2AFX / H2AX Antibody - Detection of human gamma-H2AX by western blot. Samples: Whole cell lysate (50 µg) from Jurkat cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibody: Affinity purified rabbit anti-gamma-H2AX antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
H2AFX / H2AX Antibody - Detection of Human gamma-H2AX by Western Blot. Samples: Whole cell lysate (50 ug) from 293T cells that were mock treated (-) or treated with Etoposide (100 mu M, 2h). Antibody: Affinity purified rabbit anti-gamma-H2AX antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
H2AFX / H2AX Antibody - Detection of human gamma-H2AX by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- gamma-H2AX used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB.
H2AFX / H2AX Antibody - Detection of mouse gamma-H2AX by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- gamma-H2AX used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB.
H2AFX / H2AX Antibody - Detection of Human gamma-H2AX by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-gamma-H2AX ( Lot12) used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
H2AFX / H2AX Antibody - Detection of Human gamma-H2AX by Immunocytochemistry. Samples: Neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right). Antibody: Affinity purified rabbit anti-gamma-H2AX ( Lot12) used at a dilution of 1:5000 (0.2 ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight 594 conjugated used at a dilution of 1:100.
H2AFX / H2AX Antibody - Detection of human gamma-H2AX by western blot. Samples: Whole cell lysate (50 µg) from Jurkat cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibody: Affinity purified rabbit anti-gamma-H2AX antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
H2AFX / H2AX Antibody - Detection of Human gamma-H2AX by Western Blot. Samples: Whole cell lysate (50 ug) from 293T cells that were mock treated (-) or treated with Etoposide (100 mu M, 2h). Antibody: Affinity purified rabbit anti-gamma-H2AX antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
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Polyclonal Rabbit anti‑Human H2AFX / H2AX Antibody (phospho‑Ser140, IHC, IF, WB) LS‑C287018

Polyclonal Rabbit anti‑Human H2AFX / H2AX Antibody (phospho‑Ser140, IHC, IF, WB) LS‑C287018

Antibody:
H2AFX / H2AX Rabbit anti-Human Polyclonal (pSer140) Antibody
Application:
IHC, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C287018-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
H2AFX / H2AX Rabbit anti-Human Polyclonal (pSer140) Antibody
Application:
IHC, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
H2AX antibody LS-C287018 is an unconjugated rabbit polyclonal antibody to H2AX (H2AFX) (pSer140) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB.
Target
Human H2AFX / H2AX
Synonyms
H2AFX | H2AX | Histone H2A.x | H2A.X | H2A histone family, member X | H2AX histone
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 3014 corresponding to amino acids surrounding serine 140 of UniprotID: P16104
Epitope
pSer140
Specificity
Region surrounding phosphorylated serine 140 of human histone H2AX using the numbering given in entry NP_002096.1 (GeneID 3014).
Applications
  • IHC (1:500 - 1:2000)
  • ICC (1:1000 - 1:5000)
  • Immunofluorescence (1:500 - 1:5000)
  • Western blot (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF: 1:500-1:5000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About H2AFX / H2AX
P16104 NM_002105 NP_002096.1

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Requested From: United States
Date Requested: 11/22/2024