LSBio logo with text
Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers


Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > HDAC6 > LS-C801231
 
Print Page  
Catalog Number Size Price
LS-C801231-100 100 µl (1 mg/ml) $379 
LS-C801231-200 200 µl (1 mg/ml) $421 
HDAC6 Antibody - 1:100 staining human Testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HDAC6 Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
HDAC6 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
HDAC6 Antibody - Western blot analysis of HDAC6 phosphorylation expression in NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
HDAC6 Antibody - Western blot analysis of Phospho-HDAC6 (Ser22) antibody expression in NIH-3T3 cells lysates. The lane on the right is treated with the antigen-specific peptide.
HDAC6 Antibody - 1:100 staining human Testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HDAC6 Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
HDAC6 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
HDAC6 Antibody - Western blot analysis of HDAC6 phosphorylation expression in NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
HDAC6 Antibody - Western blot analysis of Phospho-HDAC6 (Ser22) antibody expression in NIH-3T3 cells lysates. The lane on the right is treated with the antigen-specific peptide.
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5

Polyclonal Rabbit anti‑Human HDAC6 Antibody (phospho‑Ser22, IHC, IF, WB) LS‑C801231

Polyclonal Rabbit anti‑Human HDAC6 Antibody (phospho‑Ser22, IHC, IF, WB) LS‑C801231

Submit Review LSBio Guarantee
Antibody:
HDAC6 Rabbit anti-Human Polyclonal (pSer22) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801231-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HDAC6 Rabbit anti-Human Polyclonal (pSer22) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
HDAC6 antibody LS-C801231 is an unconjugated rabbit polyclonal antibody to HDAC6 (pSer22) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human HDAC6
Synonyms
HDAC6 | Histone deacetylase 6 | HD6 | KIAA0901 | JM21
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human HDAC6 around the phosphorylation site of Serine 22.
Epitope
pSer22
Specificity
Phospho-HDAC6 (Ser22) Antibody detects endogenous levels of HDAC6 only when phosphorylated at Serine 22.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HDAC6
Q9UBN7 NM_006044 NP_006035.2

Publications (0)

Submit a Publication for LS-C801231

Customer Reviews (0)

Submit a review for LS-C801231

Featured Products

HDAC6 Antibody - Anti-HDAC6 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC‑plus™ HDAC6 Antibody (aa7‑56) LS‑B5253
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
HDAC6 Antibody - Anti-HDAC6 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PathPlus™ HDAC6 Antibody (aa1100‑C‑term) LS‑B11166
Species: Human, Monkey, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Product
HDAC6 Antibody (aa1‑16) LS‑C7363
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation
HDAC6 Antibody - Anti-HDAC6 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HDAC6 Antibody (aa69‑118) LS‑B966
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
HDAC6 Antibody - Immunofluorescence analysis of HeLa cells using HDAC6 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
HDAC6 Antibody LS‑C746922
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation
HDAC6 Antibody - Western blot of HDAC-6 in NIH-3T3 cell lysate with antibody.
HDAC6 Antibody (aa1‑16) LS‑C693
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation, Chromatin Immunoprecipitation
See all HDAC6 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2024 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

To place an order:
customerservice@vectorlabs.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com