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Catalog Number Size Price
LS-C60137-100 100 µg (1.55 mg/ml) $567 
HHV-8 ORF57 Antibody - Anti-KSHV ORF57 Antibody - Western Blot. Western blot of affinity purified anti-KSHV ORF57 to detect KSHV ORF57 in HEK293 cells transfected with ORF57 expression vector and ORF57 truncations, or in KSHV infected B-cell line (BCBL-1) treated with or without valproic acid to induce viral replication (arrow). The membrane was probed with the primary antibody diluted 1:7500 (left) and 1:1000 (right). Personal Communication, V. Majerciak, M. Zheng, CCR-NCI, Bethesda, MD.

Polyclonal Rabbit anti‑Human Herpesvirus 8 HHV‑8 ORF57 Antibody (C‑Terminus, IHC, WB) LS‑C60137

Polyclonal Rabbit anti‑Human Herpesvirus 8 HHV‑8 ORF57 Antibody (C‑Terminus, IHC, WB) LS‑C60137

Antibody:
HHV-8 ORF57 Rabbit anti-Human Herpesvirus 8 Polyclonal (C-Terminus) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human Herpesvirus 8, Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C60137-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HHV-8 ORF57 Rabbit anti-Human Herpesvirus 8 Polyclonal (C-Terminus) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human Herpesvirus 8, Human
Format:
Unconjugated, Unmodified

Specifications

Description
HHV-8 ORF57 antibody LS-C60137 is an unconjugated rabbit polyclonal antibody to HHV-8 ORF57 (C-Terminus) from human herpesvirus 8. It is reactive with human and human herpesvirus 8. Validated for ELISA, IHC and WB. Cited in 1 publication.
Target
Human Herpesvirus 8 HHV-8 ORF57
Host
Rabbit
Reactivity
Human Herpesvirus 8, Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human KSHV ORF57 protein.
Epitope
C-Terminus
Specificity
A BLAST analysis was used to suggest cross-reactivity with ORF57 protein from human herpesvirus 8 types P and M sources based on 94% homology with the immunizing sequence. Reactivity against homologues from other sources is unknown.
Applications
  • IHC
  • Western blot (1:5000 - 1:8000)
  • ELISA (1:10000 - 1:70000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band 50-55 kD in size corresponding to KSHV ORF57 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

LSBio Ratings

HHV-8 ORF57 Antibody (C-Terminus) for IHC, WB/Western, ELISA LS-C60137 has an LSBio Rating of
Publications (4)

Publications (1)

Kaposi Sarcoma Herpesvirus (KSHV) Latency-Associated Nuclear Antigen (LANA) recruits components of the MRN (Mre11-Rad50-NBS1) repair complex to modulate an innate immune signaling pathway and viral latency. Mariggiò G, Koch S, Zhang G, Weidner-Glunde M, Rückert J, Kati S, Santag S, Schulz TF. PLoS pathogens. 2017 13:e1006335. (WB; Human Herpesvirus 8)

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Requested From: United States
Date Requested: 11/20/2024