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Antibodies > HNF4 Alpha+Gamma > LS-C413074
 
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Catalog Number Size Price
LS-C413074-100 100 µl (1 mg/ml) $321 
HNF4 Alpha+Gamma Antibody - Immunohistochemical analysis of HNF4 alpha/gamma staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HNF4 Alpha+Gamma Antibody - Immunofluorescent analysis of HNF4 alpha/gamma staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HNF4 Alpha+Gamma Antibody - Western blot analysis of HNF4 alpha/gamma expression in A549 (A); HepG2 (B); HeLa (C) whole cell lysates.
HNF4 Alpha+Gamma Antibody - Immunohistochemical analysis of HNF4 alpha/gamma staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HNF4 Alpha+Gamma Antibody - Immunofluorescent analysis of HNF4 alpha/gamma staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HNF4 Alpha+Gamma Antibody - Western blot analysis of HNF4 alpha/gamma expression in A549 (A); HepG2 (B); HeLa (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human HNF4 Alpha+Gamma Antibody (Internal, IHC, IF, WB) LS‑C413074

Polyclonal Rabbit anti‑Human HNF4 Alpha+Gamma Antibody (Internal, IHC, IF, WB) LS‑C413074

(4.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Antibody:
HNF4 Alpha+Gamma Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$321
LS-C413074-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Request SDS/MSDS

Overview

Antibody:
HNF4 Alpha+Gamma Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HNF4 Alpha+Gamma antibody LS-C413074 is an unconjugated rabbit polyclonal antibody to HNF4 Alpha+Gamma (Internal) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB. Cited in 1 publication.
(1) Publication(s)
Target
Human HNF4 Alpha+Gamma
Synonyms
HNF4A/HNF4G
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human HNF4 alpha/gamma.
Epitope
Internal
Specificity
Recognizes endogenous levels of HNF4 alpha/gamma protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

LSBio Ratings

HNF4 Alpha+Gamma Antibody (Internal) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-C413074 has an LSBio Rating of
(4.0) Rating
Publications (4)

Publications (1)

Embryonic liver developmental trajectory revealed by single-cell RNA sequencing in the Foxa2eGFP mouse. Tianhao Mu, Liqin Xu, Yu Zhong, Xinyu Liu, Zhikun Zhao, Chaoben Huang, Xiaofeng Lan, Chengchen Lufei, Yi Zhou, Yixun Su, Luang Xu, Miaomiao Jiang, Hongpo Zhou, Xinxin Lin, Liang Wu, Siqi Peng, Shiping Liu, Susanne Brix, Michael Dean, Norris R Dunn, Kenneth S Zaret, Xin-Yuan Fu, Yong Hou. Communications biology. 2020 November;3:642.
Full Text Article PubMed: 33144666 PMC: PMC7642341
Submit a Publication for LS-C413074

Customer Reviews (0)

Submit a review for LS-C413074

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/27/2024
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6737 Mowry Ave
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United States

Tel: (800) 227-6666 / (650) 697-3600

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