Rat HTR5A / 5-HT5A Receptor

Rabbit

Rat, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide sequence corresponding to aa17-34 of the rat 5-HT5A receptor.

aa17-34

Recognizes Serotonin Receptor 5A. Species cross-reactivity: Mouse and rat.

• IHC (1:100 - 1:300)
• Immunofluorescence
• Western blot (1:100 - 1:250)

Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:100-1:250. Immunohistochemistry: 1:100-1:300 in PBS/0.03% Triton X-100 - Bn/Av-HRP Technique. Control Tissue: The antiserum demonstrates positive labeling of rat cortex and hippocampus. Perfusion Fixation: Fixative-4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 ml over approximately 20 minutes. Post Fixation: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 ml over approximately 20 minutes. Post Fixation -1.5 hour at 4? C. in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.10.3%) may be used in conjunction with paraformaldehyde. Sections: 50um vibratome. Absorption control: Synthetic rat 5HT 5A receptor. Incubation on Tissue: 16-24 hours at 4?C.

Supplied as a liquid.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P47898 NCBI: NM_024012 NP_076917.1