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Catalog Number Size Price
LS-C352341-100 100 µl (1 mg/ml) $299 
IAPP / Amylin Antibody - Immunohistochemical analysis of Amylin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
IAPP / Amylin Antibody - Immunofluorescent analysis of Amylin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IAPP / Amylin Antibody - Western blot analysis of Amylin expression in MCF7 (A); HeLa (B); mouse kidney (C); rat kidney (D) whole cell lysates.
IAPP / Amylin Antibody - Immunohistochemical analysis of Amylin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
IAPP / Amylin Antibody - Immunofluorescent analysis of Amylin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IAPP / Amylin Antibody - Western blot analysis of Amylin expression in MCF7 (A); HeLa (B); mouse kidney (C); rat kidney (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human IAPP / Amylin Antibody (N‑Terminus, IHC, IF, WB) LS‑C352341

Polyclonal Rabbit anti‑Human IAPP / Amylin Antibody (N‑Terminus, IHC, IF, WB) LS‑C352341

Antibody:
IAPP / Amylin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352341-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IAPP / Amylin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
Amylin antibody LS-C352341 is an unconjugated rabbit polyclonal antibody to Amylin (IAPP) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Cited in 2 publications.
Target
Human IAPP / Amylin
Synonyms
IAPP | Diabetes-associated peptide | Islet amyloid polypeptide | AMYLIN | IAP | Insulinoma amyloid peptide
Host
Rabbit
Reactivity
Human, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human Amylin.
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of Amylin protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IAPP / Amylin
P10997 NM_000415 NP_000406.1

LSBio Ratings

IAPP / Amylin Antibody (N-Terminus) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-C352341 has an LSBio Rating of
Publications (4.1)

Publications (2)

Melatonin influences the expression and oligomerization of amylin in rat INS-1E cells. E-M Jung, Y-M Yoo, E-B Jeung. Journal of physiology and pharmacology : an official journal of the Polish Physiological Society. 2019 October;70:

Human Islet Amyloid Polypeptide Overexpression in INS-1E Cells Influences Amylin Oligomerization under ER Stress and Oxidative Stress. Yeong-Min Yoo, Seong Soo Joo. International journal of molecular sciences. 2021 Oct;22:11341.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/22/2024