Human IGFBP5

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Biotin. Also available Unconjugated.

Immunoaffinity purified

Unmodified

E.coli derived recombinant Human IGF-BP5.

Human IGF-BP5

• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.25 - 1 µg/ml)
• Applications tested for the base form of this product only

ELISA: To detect hIGF-BP5 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP5. To detect hIGF-BP5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IGF-BP5 (LS-B8207) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP5. Western Blot: To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, pH 7.2

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P24593 NCBI: NM_000599 NP_000590.1