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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2977-50 | 50 µg (1 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human ACTH Antibody (clone 57, N‑Terminus, IHC) LS‑B2977
IHC‑plus™ Monoclonal Mouse anti‑Human ACTH Antibody (clone 57, N‑Terminus, IHC) LS‑B2977
Note: This antibody replaces LS-C11780, LS-C391681
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
ACTH antibody LS-B2977 is an unconjugated mouse monoclonal antibody to ACTH (N-Terminus) from human. It is reactive with human and rat. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Host
Mouse
Reactivity
Human, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
57
Purification
Protein A purified
Modifications
Unmodified
Immunogen
ACTH (KLH). Epitope: N-Terminus.
Epitope
N-Terminus
Specificity
Recognizes human Synacthen (1-24 ACTH). No crossreactivity to CLIP (17-39 ACTH). Species Crossreactivity: rat
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2977 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2977 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.6, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Anti-ACTH antibody IHC of human pituitary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ACTH antibody IHC of human pituitary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ ACTH Antibody (N-Terminus, clone 57) for IHC, ELISA LS-B2977 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, ICC, ELISA
Species:
Human, Mouse, Rat, Pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
Date Requested: 12/21/2024
