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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3882-50 | 50 µl | $440 |
1 of 2
2 of 2
IHC‑plus™ Monoclonal Mouse anti‑Human ALCAM / CD166 Antibody (clone 3F8B12, IHC, WB) LS‑B3882
IHC‑plus™ Monoclonal Mouse anti‑Human ALCAM / CD166 Antibody (clone 3F8B12, IHC, WB) LS‑B3882
Note: This antibody replaces LS-C108764
Antibody:
ALCAM / CD166 Mouse anti-Human Monoclonal (3F8B12) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
ALCAM / CD166 Mouse anti-Human Monoclonal (3F8B12) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CD166 antibody LS-B3882 is an unconjugated mouse monoclonal antibody to human CD166 (ALCAM). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ALCAM / CD166
Synonyms
ALCAM | CD166 antigen | MEMD | CD166
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
3F8B12
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant fragment of ALCAM (aa405-524) expressed in E. Coli.
Specificity
Human
Applications
- IHC
- IHC - Paraffin (1:200 - 1:400)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3882 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3882 was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ALCAM / CD166
Validation
Anti-ALCAM / CD166 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-ALCAM / CD166 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
See More About...
LSBio Ratings
IHC-plus™ ALCAM / CD166 Antibody (clone 3F8B12) for IHC, WB/Western, ELISA LS-B3882 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
Flow Cytometry, Functional Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Species:
Mouse, Human, Rat
Applications:
ICC, Western blot, Immunoprecipitation
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/7/2024
Date Requested: 5/7/2024
