IHC‑plus™ Monoclonal Rat anti‑Human APAF1 / APAF‑1 Antibody (clone 2E12, Low Endotoxin, aa1‑464, IHC, WB) LS‑B12884
IHC‑plus™ Monoclonal Rat anti‑Human APAF1 / APAF‑1 Antibody (clone 2E12, Low Endotoxin, aa1‑464, IHC, WB) LS‑B12884
Note: This antibody replaces LS-C344792
Antibody:
APAF1 / APAF-1 Rat anti-Human Monoclonal (aa1-464) (Low Endotoxin) (2E12) Antibody
Application:
IHC, IHC-P, ICC, WB, IP, ELISA
Format:
Unconjugated, Low Endotoxin
Overview
Antibody:
APAF1 / APAF-1 Rat anti-Human Monoclonal (aa1-464) (Low Endotoxin) (2E12) Antibody
Application:
IHC, IHC-P, ICC, WB, IP, ELISA
Format:
Unconjugated, Low Endotoxin
Specifications
Description
APAF-1 antibody LS-B12884 is an unconjugated rat monoclonal antibody to human APAF-1 (APAF1) (aa1-464). Validated for ELISA, ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human APAF1 / APAF-1
Synonyms
APAF1 | APAF-1 | CED4 | KIAA0413
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Low Endotoxin
Immunogen
Recombinant human Apaf-1 (aa 1-464) containing the N-Terminus CARD and CED-4 homologous domains.
Specificity
Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- ICC
- Western blot (1:1000 - 1:2000)
- Immunoprecipitation (1:1000 - 1:2000)
- ELISA
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry - EM on frozen sections, see G. Hausmann, et al.. (2000. Immunoprecipitation - 1:1000-1:2000. Western Blot - 1:1000-1:2000. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, 0.02% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human, Mouse, Rat, Gerbil
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin
Species:
Human, Mouse, Rat
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Reactivity:
Rat
Range:
0.156-10 ng/ml
Source:
Human
Tag:
8His, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024