Target
Human APOA1 / Apolipoprotein A 1
Synonyms
APOA1 | ApoA-I | APOAI | Apolipoprotein A-I | Apolipoprotein A1 | Apo-AI
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Highly purified Apolipoprotein Type A-I from human plasma
Specificity
Recognizes human Apolipoprotein A-I; minimum cross-reactivity against Type A-II, B, C-I, C-II, C-III, E and J Apolipoproteins and other human serum proteins.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:2500 - 1:5000)
- Immunoprecipitation
- ELISA (1:5000 - 1:10000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2475 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2475 was determined to be 5 ug/ml.
Presentation
BBS, pH 8.0, 0.005 M EDTA, 0.01% sodium azide, 40% glycerol. No stabilizing proteins added.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APOA1 / Apolipoprotein A 1