Human B2M / Beta 2 Microglobulin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Ion exchange chromatography

Unmodified

Purified human b2-microglobulin.

Recognizes human b2-microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as soluble human b2-microglobulin. Does not cross-react with mouse, bovine, rabbit, dog or chicken b2-microglobulin.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (2 - 4 µg/ml)
• Immunoprecipitation
• Flow Cytometry (1 µg/ml)
• ELISA

Immunohistochemistry: LS-B2426 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2426 was determined to be 10 ug/ml.

PBS, pH 7.4, 15 mM Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P61769 NCBI: NM_004048 NP_004039.1