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| Catalog Number | Size | Price |
|---|---|---|
| LS-B499-0.1 | 0.1 ml (0.9 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human BIRC5 / Survivin Antibody (clone 60.11, IHC, WB) LS‑B499
IHC‑plus™ Monoclonal Mouse anti‑Human BIRC5 / Survivin Antibody (clone 60.11, IHC, WB) LS‑B499
Note: This antibody replaces LS-C2904
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
Survivin antibody LS-B499 is an unconjugated mouse monoclonal antibody to Survivin (BIRC5) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BIRC5 / Survivin
Synonyms
BIRC5 | API4 | Apoptosis inhibitor survivin | Apoptosis inhibitor 4 | Survivin | Survivin variant 3 alpha | EPR-1 | IAP4
Host
Mouse
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Clone
60.11
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human survivin, full-length
Specificity
the cytoplasmic form of survivin.
Applications
- IHC
- IHC - Paraffin (2.5 - 5 µg/ml)
- Western blot (1:1000 - 1:3000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B499 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B499 was determined to be 2.5-5 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About BIRC5 / Survivin
Validation
Anti-BIRC5 / Survivin antibody IHC of human ovarian cancer. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BIRC5 / Survivin antibody IHC of human ovarian cancer. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ BIRC5 / Survivin Antibody (clone 60.11) for IHC, WB/Western LS-B499 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/28/2024
Date Requested: 11/28/2024
