Human BMI1 / PCGF4

Goat

Human (tested or 100% immunogen sequence identity)

Human, Chimpanzee, Orangutan, Mouse, Rat, Bovine, Dog (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 252-264 of human Bmi1 protein.

A BLAST analysis was used to suggest cross reactivity with Bmi1 protein from human, rat, orangutan, dog, chimpanzee, bovine and cat based on 100% homology to the immunogen sequence. This sequence shows significant homology to homologues from Xenopus and chicken sources. Reactivity against homologues from other sources is not known.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Immunofluorescence (1:200)
• Western blot (1:500 - 1:3000)
• ELISA (1:5000 - 1:30000)

Immunohistochemistry: LS-B346 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B346 was determined to be 2.5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Expiration date is one year from opening.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P35226 NCBI: NM_005180 NP_005171.4