Human CALD1 / Caldesmon

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Recombinant Monoclonal

CALD1/1424R

Unconjugated

Protein A/G purified

Unmodified. Also available Azide-free, Carrier-free.

Recombinant full-length human CALD1 protein. Antigen Molecular Weight: 150 kDa

Recognizes a protein of 150 kDa , which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa ) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa ) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

• IHC - Paraffin (1:100)

Immunohistology (Formalin-fixed - 1-2 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS, 0.05% BSA, 0.05% Sodium Azide

Store at 2°C to 8°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q05682 NCBI: NM_004342 NP_004333.1