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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2021-100 | 100 µl (0.5 mg/ml) | $460 |
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IHC‑plus™ Polyclonal Rabbit anti‑Rat CASP9 / Caspase 9 Antibody (aa345‑359, IHC, WB) LS‑B2021
IHC‑plus™ Polyclonal Rabbit anti‑Rat CASP9 / Caspase 9 Antibody (aa345‑359, IHC, WB) LS‑B2021
Note: This antibody replaces LS-C647
Antibody:
CASP9 / Caspase 9 Rabbit anti-Rat Polyclonal (aa345-359) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Rat, Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CASP9 / Caspase 9 Rabbit anti-Rat Polyclonal (aa345-359) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Rat, Human
Format:
Unconjugated, Unmodified
Specifications
Description
Caspase 9 antibody LS-B2021 is an unconjugated rabbit polyclonal antibody to Caspase 9 (CASP9) (aa345-359) from rat. It is reactive with human and rat. Validated for IHC and WB.
Target
Rat CASP9 / Caspase 9
Synonyms
CASP9 | APAF3 | CASPASE-9c | ICE-LAP6 | MCH6 | ICE-like apoptotic protease 6 | APAF-3 | Apoptotic protease Mch-6 | CASP-9 | Caspase 9 | Caspase-9 | PPP1R56
Host
Rabbit
Reactivity
Rat, Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A mixture of synthetic peptides corresponding to amino acids 345-359 (CLFPSPPQSLTASMG) and 366-383 (GTRHWYMYSRTSTSPPKA) of rat Caspase-9 (CTD). Percent identity by BLAST analysis: Rat (100%).
Epitope
aa345-359
Specificity
A 45 kD band is detected.
Applications
- IHC
- IHC - Paraffin (1:200) & (5 µg/ml)
- Western blot (1 - 2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2021 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B2021 was determined to be 1:200.
Presentation
PBS, 0.05% Sodium Azide, 0.05% BSA
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CASP9 / Caspase 9
Validation
Anti-Caspase 9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ CASP9 / Caspase 9 Antibody (aa345-359) for IHC, WB/Western LS-B2021 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Dog, Gerbil
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Species:
Human, Mouse, Rat, Dog
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Reactivity:
Human
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/24/2024
Date Requested: 12/24/2024
