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| Catalog Number | Size | Price |
|---|---|---|
| LS-A9435-50 | 50 µg (1 mg/ml) | $460 |
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2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human CD45 / LCA Antibody (Internal, IHC) LS‑A9435
IHC‑plus™ Polyclonal Rabbit anti‑Human CD45 / LCA Antibody (Internal, IHC) LS‑A9435
Antibody:
CD45 / LCA Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Overview
Antibody:
CD45 / LCA Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
LCA antibody LS-A9435 is an unconjugated rabbit polyclonal antibody to human LCA (CD45) (Internal). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human CD45 / LCA
Synonyms
PTPRC | B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 16 amino acid peptide from internal region of human CD45. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon (88%).
Epitope
Internal
Specificity
Human CD45. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
- IHC
- IHC - Paraffin (2.5 - 5 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5-5 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD45 / LCA
Validation
Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 2.5-5 ug/ml.
Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 2.5-5 ug/ml.
Anti-CD45 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 2.5-5 ug/ml.
Anti-CD45 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 2.5-5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ CD45 / LCA Antibody (Internal) for IHC LS-A9435 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
Date Requested: 12/22/2024
