Target
Human CD68
Synonyms
CD68 | CD68 antigen | gp110 | SCARD1 | CD68 molecule | LAMP4 | Macrophage antigen CD68 | Macrosialin
Reactivity
Human, Monkey, Rat, Cat
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified from ascites
Immunogen
Subcellular fraction of human alveolar macrophages. Cellular Localization: Cytoplasmic.
Specificity
Recognizes the human glycoprotein of 110kD, which is identified as CD68. Species cross-reactivity: Monkey, feline, rat. Does not react with porcine, canine, chicken.
Applications
- IHC
- IHC - Paraffin (1:50)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
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Usage
Immunohistochemistry: LS-B2862 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2862 was determined to be 1:50. Positive control: Tonsil, lymph node or spleen.
Presentation
10 mM PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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