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Catalog Number Size Price
LS-B2820-0.05 0.05 mg (1 mg/ml) $460 
CD95 / FAS Antibody - Anti-FAS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CD95 / FAS Antibody - Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE).  Histograms:  Red – Isotype mouse IgG1 control staining  Black – standard anti-CD95 monoclonal antibody (DX2)  Green – anti-CD95 (LT95)    A – JURKAT human peripheral blood T cell leukemia cell line  B – RAMOS human Burkitt lymphoma cell line  C – CEM human leukemia cell line  D – MOLT-4 human acute lymphoblastic T cell leukemia cell line
CD95 / FAS Antibody - Anti-FAS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CD95 / FAS Antibody - Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE).  Histograms:  Red – Isotype mouse IgG1 control staining  Black – standard anti-CD95 monoclonal antibody (DX2)  Green – anti-CD95 (LT95)    A – JURKAT human peripheral blood T cell leukemia cell line  B – RAMOS human Burkitt lymphoma cell line  C – CEM human leukemia cell line  D – MOLT-4 human acute lymphoblastic T cell leukemia cell line
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IHC‑plus™ Monoclonal Mouse anti‑Human CD95 / FAS Antibody (clone LT95, IHC) LS‑B2820

IHC‑plus™ Monoclonal Mouse anti‑Human CD95 / FAS Antibody (clone LT95, IHC) LS‑B2820

Note: This antibody replaces LS-C46014
Antibody:
CD95 / FAS Mouse anti-Human Monoclonal (LT95) Antibody
Application:
IHC, IHC-P, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$460
LS-B2820-0.05
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD95 / FAS Mouse anti-Human Monoclonal (LT95) Antibody
Application:
IHC, IHC-P, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
FAS antibody LS-B2820 is an unconjugated mouse monoclonal antibody to human FAS (CD95). Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human CD95 / FAS
Synonyms
FAS | ALPS1A | APO-1 | APT1 | Apo-1 antigen | APO-1 cell surface antigen | Apo11 antigen | Apoptosis antigen 1 | CD95 antigen | Delta Fas/APO-1/CD95 | Fas AMA | FAS1 | Fasl receptors | FASLG receptor | FASTM | FAS 827dupA | Fasl receptor | TNFRSF6 | CD95
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
LT95
Conjugations
Unconjugated. Also available conjugated with FITC, PE.
Purification
Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
Modifications
Unmodified
Immunogen
HUT-78 human T cell lymphoma cell line
Specificity
The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumor necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD95 / FAS
P25445 NM_152877 NP_000034.1

Validation

CD95 / FAS Antibody - Anti-FAS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-FAS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-FAS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ CD95 / FAS Antibody (clone LT95) for IHC, Flow LS-B2820 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Combined evaluation of the FAS cell surface death receptor and CD8+ tumor infiltrating lymphocytes as a prognostic biomarker in breast cancer. Blok EJ, van den Bulk J, Dekker-Ensink NG, Derr R, Kanters C, Bastiaannet E, Kroep JR, van de Velde CJ, Kuppen PJ. Oncotarget. 2017 8:15610-15620. (Flo; Human)

Customer Reviews (0)


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Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/21/2024