Human CXCL1 / GRO Alpha

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Highly pure (>98%) recombinant hGRO/MGSA (human GRO/MGSA)

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.2 µg/ml)
• Neutralization (1.5 - 3 µg/ml)

Immunohistochemistry: LS-B2843 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2843 was determined to be 5 ug/ml. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO/MGSA 100 ng/ml), a concentration of 1.5 - 3.0 ug/ml of this antibody is required. ELISA: To detect hGRO/MGSA by direct ELISA (using 100 ul/well antibody solution) a concentration of at least 0.5 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO/MGSA. Western Blot: To detect hGRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO/MGSA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Lyophilized from PBS, pH 7.2

Reconstitute in sterile water to 0.1-1 mg/ml.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P09341 NCBI: NM_001511 NP_001502.1