Mouse DLL1

Rabbit

Mouse, Human, Rat, Chicken (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 150-175 of Mouse DELTA-1.  Swiss Prot accession Q61483.

The antibody detects DELTA-1 from mouse. Activity against Delta-1 from other species has not been determined but is expected based on sequence homology. Expected to cross-react with rat, human and chicken.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (1:100 - 1:1000)
• ELISA (1:3000 - 1:12000)

Immunohistochemistry: LS-B72 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B72 was determined to be 10 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Aliquot and store at -20°C or below for extended storage. Avoid freeze / thaw cycles. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O00548 NCBI: NM_005618 NP_005609.3