Target
Human EMA / MUC1
Synonyms
MUC1 | CA15-3 | CD227 | Carcinoma-associated mucin | CD227 antigen | DF3 antigen | Episialin | Epithelial membrane antigen | H23 antigen | H23AG | Krebs von den Lungen-6 | MAM6 | MUC1/ZD | MUC-1/SEC | Mucin-1 | Pem | KL-6 | MUC-1/X | Tumor-associated mucin | Peanut-reactive urinary mucin | Polymorphic epithelial mucin | PUM | EMA | MUC-1 | Mucin 1, transmembrane
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG3
Monoclonal
Purification
Protein G purified
Immunogen
Urinary MUC-1 mucin.
Specificity
Recognizes human Mucin 1. Recognizes the peptide epitope Arg-Pro-Ala-Pro within the protein core of the mucin.
Applications
- IHC
- IHC - Paraffin (1:50)
- Immunofluorescence
- Flow Cytometry
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2244 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2244 was determined to be 1:50. Flow Cytometry: Neat-1:10. Use 10 ul to label 10^6 cells in 100ul. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 1% BSA
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee