Human EPCAM

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

HEA-125

Unconjugated

Purified IgG

Unmodified

HT29 carcinoma cell line.

Recognizes the 34kD cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM). This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumor specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, and de novo expression being seen in dysplastic squamous epithelium.

• IHC
• IHC - Paraffin (1:50)
• IHC - Frozen (1:20)
• Immunofluorescence
• Flow Cytometry (1:1)

Flow Cytometry: Use 5 ul of the suggested working dilution to label 10^6 cells or 100 ul cell suspension. Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin sections e. g. 0.1% pronase, 10 minutes. Histology Positive Control Tissue: Human colon & appendix.

PBS, 0.09% Sodium Azide, 0.5% BSA

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16422 NCBI: NM_002354 NP_002345.2