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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3904-50 | 50 µl | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human EPHB2 / EPH Receptor B2 Antibody (clone 2D12C6, aa17‑200, IHC, IF, WB) LS‑B3904
IHC‑plus™ Monoclonal Mouse anti‑Human EPHB2 / EPH Receptor B2 Antibody (clone 2D12C6, aa17‑200, IHC, IF, WB) LS‑B3904
Note: This antibody replaces LS-C108801
Antibody:
EPHB2 / EPH Receptor B2 Mouse anti-Human Monoclonal (aa17-200) (2D12C6) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
EPHB2 / EPH Receptor B2 Mouse anti-Human Monoclonal (aa17-200) (2D12C6) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
EPH Receptor B2 antibody LS-B3904 is an unconjugated mouse monoclonal antibody to human EPH Receptor B2 (EPHB2) (aa17-200). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human EPHB2 / EPH Receptor B2
Synonyms
EPHB2 | CAPB | Cek5 | EK5 | ETECK | EPH tyrosine kinase 3 | DRT | ELK-related tyrosine kinase | Hek5 | EPH-like kinase 5 | EPHT3 | Nuk | PCBC | Tyrosine-protein kinase TYRO5 | Protein-tyrosine kinase HEK5 | Tyro5 | EPH receptor B2 | Ephrin type-B receptor 2 | EPTH3
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Clone
2D12C6
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant fragment of EphB2 (aa17-200) expressed in E. Coli.
Epitope
aa17-200
Specificity
Human
Applications
- IHC
- IHC - Paraffin (1:200 - 1:400)
- Immunofluorescence (1:200 - 1:1000)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3904 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3904was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About EPHB2 / EPH Receptor B2
Validation
Anti-EPHB2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
See More About...
LSBio Ratings
IHC-plus™ EPHB2 / EPH Receptor B2 Antibody (aa17-200, clone 2D12C6) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B3904 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
Western blot, Immunoprecipitation, ELISA
Species:
Human, Monkey, Mouse, Rat, Rabbit, Zebrafish
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Mouse, Human
Applications:
Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin
Reactivity:
Human
Range:
0.625-40 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
