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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2321-50 | 50 µg (0.5 mg/ml) | $485 |
IHC‑plus™ Monoclonal Mouse anti‑Human F2RL1 / PAR2 Antibody (clone SAM11, N‑Terminus, IHC, IF) LS‑B2321
IHC‑plus™ Monoclonal Mouse anti‑Human F2RL1 / PAR2 Antibody (clone SAM11, N‑Terminus, IHC, IF) LS‑B2321
Note: This antibody replaces LS-C38064
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
PAR2 antibody LS-B2321 is an unconjugated mouse monoclonal antibody to human PAR2 (F2RL1) (N-Terminus). Validated for ELISA, IF and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human F2RL1 / PAR2
Synonyms
F2RL1 | PAR2 | Thrombin receptor-like 1 | Protease-activated receptor 2 | G-protein coupled receptor 11 | GPR11 | PAR-2
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
SAM11
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to the N-Terminus of human PAR2 receptor.
Epitope
N-Terminus
Specificity
Recognizes native peptide and the N-terminus of the human PAR2 receptor. Does not cross-react with PAR1.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence (1 - 3 µg/ml)
- ELISA (0.1 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2321 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2321 was determined to be 5 ug/ml. Immunofluorescence: 1-3 ug/ml. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About F2RL1 / PAR2
Validation
Anti-F2RL1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-F2RL1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ F2RL1 / PAR2 Antibody (N-Terminus, clone SAM11) for IHC, IF/Immunofluorescence, ELISA LS-B2321 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
PAR2-dependent activation of GSK3ß regulates the survival of colon stem/progenitor cells. Nasri I, Bonnet D, Zwarycz B, d'Aldebert E, Khou S, Mezghani-Jarraya R, Quaranta M, Rolland C, Bonnart C, Mas E, Ferrand A, Cenac N, Magness S, Van Landeghem L, Vergnolle N, Racaud-Sultan C. American journal of physiology. Gastrointestinal and liver physiology. 2016 311:G221-36. (WB; Mouse)
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
