Human Fibrinogen

Sheep

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with FITC.

Protein G purified

Unmodified

Human Fibrinogen purified from plasma.

Recognizes human fibrinogen, a complex ~340kD hetero-hexameric (di-trimeric) glycoprotein consisting of 3 pairs of alpha, beta and gamma chains linked by a series of 29 disulphide bonds. The six chains are arranged in such a way that all the N-Terminal ends adjoin to form a central with two trimeric coiled coil structures connecting to outer D domains. Fibrinogen plays an important role in the coagulation process with the D and E domains interacting via the C-Terminal ends of the alpha chains during fibrin clot cross-linking. Shows minimal cross-reactivity with related serum proteins. Fibrinogen has been identified as a ferritin binding protein in the horse. Has been successfully as a capture reagent for ferritin - anti ferritin IgG complexes in horse plasma to evaluate the antibody response to ferritin by ELISA.

• IHC
• IHC - Paraffin (5 µg/ml)
• IHC - Frozen
• Immunofluorescence (1:200 - 1:400)
• ELISA (1:5000 - 1:50000)

Immunohistochemistry: LS-B2573 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2573 was determined to be 5 ug/ml.

Glycine buffered saline, <0.1% sodium azide, <0.01% Benzamidine, <1mM EDTA

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.