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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3587-50 | 50 µl (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human G6PD Antibody (clone AT2F6, IHC, WB) LS‑B3587
IHC‑plus™ Monoclonal Mouse anti‑Human G6PD Antibody (clone AT2F6, IHC, WB) LS‑B3587
Note: This antibody replaces LS-C103494
Antibody:
G6PD Mouse anti-Human Monoclonal (AT2F6) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
G6PD Mouse anti-Human Monoclonal (AT2F6) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
G6PD antibody LS-B3587 is an unconjugated mouse monoclonal antibody to human G6PD. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Clone
AT2F6
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human G6PD (35-506aa) purified from E. coli
Specificity
human G6PD (35-506aa)
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:1000 - 1:2000)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3587 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3587 was determined to be 5 ug/ml. Western Blot: Cell lysates of MCF7 (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human G6PD (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About G6PD
Validation
Anti-G6PD antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-G6PD antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-G6PD antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-G6PD antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ G6PD Antibody (clone AT2F6) for IHC, WB/Western, ELISA LS-B3587 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 10/31/2024
Date Requested: 10/31/2024
