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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1781-50 | 50 µg (0.5 mg/ml) | $460 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human HCAR3 / GPR109B / HM74 Antibody (aa1‑50, IHC, WB) LS‑B1781
IHC‑plus™ Polyclonal Rabbit anti‑Human HCAR3 / GPR109B / HM74 Antibody (aa1‑50, IHC, WB) LS‑B1781
Note: This antibody replaces LS-C47293
Antibody:
HCAR3 / GPR109B / HM74 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Dog, Hamster
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
HCAR3 / GPR109B / HM74 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Dog, Hamster
Format:
Unconjugated, Unmodified
Specifications
Description
HM74 antibody LS-B1781 is an unconjugated rabbit polyclonal antibody to HM74 (HCAR3 / GPR109B) (aa1-50) from human. It is reactive with human, dog and hamster. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HCAR3 / GPR109B / HM74
Synonyms
HCAR3 | GPR109B | GTP-binding protein | NIACR2 | HM74 | HM74b | Nicotinic acid receptor 2 | Putative chemokine receptor | Niacin receptor 2 | Puma-g | HCA3 | PUMAG
Host
Rabbit
Reactivity
Human, Dog, Hamster
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A portion of amino acids 1-50of human GPR109B.
Epitope
aa1-50
Specificity
A portion of amino acids 1-50 of human GPR109B.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (0.5 - 2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1781 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1781 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HCAR3 / GPR109B / HM74
Validation
Anti-GPR109B / HM74 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-GPR109B / HM74 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ HCAR3 / GPR109B / HM74 Antibody (aa1-50) for IHC, WB/Western LS-B1781 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC
Species:
Human
Applications:
Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human, Dog, Hamster
Applications:
IHC, IHC - Paraffin, ICC
Reactivity:
Human
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/1/2024
Date Requested: 11/1/2024
