Human ICAM-1 / CD54

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with Biotin, FITC, HRP, PE.

Protein G purified

Unmodified

Human monocytes

Recognizes the human CD54 cell surface antigen, a 90kD glycoprotein also known as ICAM-1. CD54 is expressed by many cells following activation by inflammatory mediators. Clone 3H1547 blocks CD54 function.

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen (1:50 - 1:100)
• Immunoprecipitation
• Flow Cytometry (1:50 - 1:100)

Immunohistochemistry: LS-B2647 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2647 was determined to be 10 ug/ml. Flow Cytometry: 1:50-1:100. Use 10 ul to label 10^6 cells in 100ul. Positive control: Human Tonsil.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05362 NCBI: NM_000201 NP_000192.2