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Catalog Number Size Price
LS-B8192-500 500 µg (1 mg/ml) $460 
Leptin Antibody - Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)
Leptin Antibody - Anti-Human Leptin Western Blot Reduced
Leptin Antibody - Anti-Human Leptin Western Blot Unreduced
Leptin Antibody - Anti-Human Leptin Sandwich ELISA
Leptin Antibody - Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)
Leptin Antibody - Anti-Human Leptin Western Blot Reduced
Leptin Antibody - Anti-Human Leptin Western Blot Unreduced
Leptin Antibody - Anti-Human Leptin Sandwich ELISA
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IHC‑plus™ Monoclonal Mouse anti‑Human Leptin Antibody (IHC, WB) LS‑B8192

IHC‑plus™ Monoclonal Mouse anti‑Human Leptin Antibody (IHC, WB) LS‑B8192

Note: This antibody replaces LS-C104300, LS-C54171, LS-C89454, LS-C104734, LS-C25159
Antibody:
Leptin Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B8192-500
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Leptin Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Leptin antibody LS-B8192 is an unconjugated mouse monoclonal antibody to human Leptin. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Leptin
Synonyms
LEP | Leptin | LEPD | Obese protein | Obese, mouse, homolog of | Obesity factor | OBS | OB
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human Leptin.
Specificity
Human Leptin
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 0.4 µg/ml)
  • ELISA (12 - 16 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B8192 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect recombinant human Leptin when used with biotinylated antigen affinity purified anti-human Leptin as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 2-4 ng/lane, under non-reducing conditions.
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Leptin
P41159 NM_000230 NP_000221.1

Validation

Leptin Antibody - Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ Leptin Antibody for IHC, WB/Western, ELISA LS-B8192 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/22/2024