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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2925-50 | 50 µl (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human MIF Antibody (clone 4E4, IHC, WB) LS‑B2925
IHC‑plus™ Monoclonal Mouse anti‑Human MIF Antibody (clone 4E4, IHC, WB) LS‑B2925
Note: This antibody replaces LS-C93767
Antibody:
MIF Mouse anti-Human Monoclonal (4E4) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
MIF Mouse anti-Human Monoclonal (4E4) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
MIF antibody LS-B2925 is an unconjugated mouse monoclonal antibody to human MIF. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MIF
Synonyms
MIF | GLIF | L-dopachrome tautomerase | L-dopachrome isomerase | MMIF | Phenylpyruvate tautomerase
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
4E4
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human MIF (1-115 aa) purified from E. coli.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:500 - 1:2000)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2925 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2925 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MIF
Validation
Anti-MIF antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MIF antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ MIF Antibody (clone 4E4) for IHC, WB/Western, ELISA LS-B2925 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Monkey
Applications:
IHC, IHC - Paraffin, Western blot, ELISA, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
Date Requested: 12/21/2024
