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Catalog Number	Size	Price
LS-B6695-500	500 µg	$460

MPO / Myeloperoxidase Antibody - Anti-MPO / Myeloperoxidase antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

MPO / Myeloperoxidase Antibody - Rabbit antibody to MPO. Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to MPO: red; Hoechst: blue; MHC class II: green. Magnification: 200 X

MPO / Myeloperoxidase Antibody - Rabbit antibody to MPO. Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 200X

MPO / Myeloperoxidase Antibody - Rabbit antibody to MPO. Isolated monocytes were stained with red followed by staining with Rabbit antibody (3 ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 80X

MPO / Myeloperoxidase Antibody - Rabbit antibody to MPO. 10 ug of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane and blocked by incubation with PBS containing 2% skim milk and 0.02% Tween 20 for 30 min. Membranes were probed with Rabbit antibody to MPO (3 ug/ml) for 1 hr. Membranes were then probed with goat anti-rabbit antibody conjugated to alkaline phosphatase diluted 1:1000 and developed with ECF luminescence substrate (Amersham Biosciences).

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IHC‑plus™ Polyclonal Rabbit anti‑Human MPO / Myeloperoxidase Antibody (IHC, IF, WB) LS‑B6695

Name: IHC-plus™ MPO / Myeloperoxidase Polyclonal Antibody Rabbit | LSBio
Brand: Lifespan BioSciences
SKU: LS-B6695-500
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

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Note: This antibody replaces LS-C93836

Antibody:

MPO / Myeloperoxidase Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, IHC-Fr, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$460

LS-B6695-500

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

MPO / Myeloperoxidase Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, IHC-Fr, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Myeloperoxidase antibody LS-B6695 is an unconjugated rabbit polyclonal antibody to human Myeloperoxidase (MPO). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human MPO / Myeloperoxidase

Synonyms

MPO | Myeloperoxidase precursor | Myeloperoxidase

See All MPO / Myeloperoxidase Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Protein G purified

Modifications

Unmodified

Immunogen

Native myeloperoxidase isolated from human leucocytes.

Specificity

Specific for MPO.

Applications

IHC
IHC - Paraffin (2.5 µg/ml)
IHC - Frozen
Immunofluorescence
Western blot (10 - 50 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

IHC: This antibody performs superbly in paraffin-embedded tissue sections fixed in formalin, frozen sections and cell cytospins. Antigen retrieval is essential for use on paraffin sections.

Presentation

Lyophilized from PBS

Reconstitution

500 µl Sterile water

Storage

Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MPO / Myeloperoxidase

Uniprot: P05164 NCBI: NM_000250 NP_000241.1

Validation

Anti-MPO / Myeloperoxidase antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

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