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Catalog Number Size Price
LS-B8354-50 50 µg (0.5 mg/ml) $485 
NQO1 Antibody - Anti-NQO1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
NQO1 Antibody - NQO1 antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
NQO1 Antibody - NQO1 antibody (0.3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
NQO1 Antibody - NQO1 antibody (0.3 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NQO1 Antibody - Anti-NQO1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
NQO1 Antibody - NQO1 antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
NQO1 Antibody - NQO1 antibody (0.3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
NQO1 Antibody - NQO1 antibody (0.3 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human NQO1 Antibody (aa134‑146, IHC, IF, WB) LS‑B8354

IHC‑plus™ Polyclonal Goat anti‑Human NQO1 Antibody (aa134‑146, IHC, IF, WB) LS‑B8354

Note: This antibody replaces LS-C139757
Antibody:
NQO1 Goat anti-Human Polyclonal (aa134-146) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B8354-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
NQO1 Goat anti-Human Polyclonal (aa134-146) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NQO1 antibody LS-B8354 is an unconjugated goat polyclonal antibody to human NQO1 (aa134-146). Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NQO1
Synonyms
NQO1 | Azoreductase | DHQU | DT-diaphorase | Dioxin-inducible 1 | DIA4 | Diaphorase-4 | NAD(P)H:quinone oxireductase | Phylloquinone reductase | QR1 | Menadione reductase | NMOR1 | NMORI | DTD | Quinone reductase 1
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-DKGPFRSKKAVLS, from the internal region of the protein sequence according to NP_000894.1.
Epitope
aa134-146
Specificity
Human NQO1. This antibody is expected to recognize reported isoform a (NP_000894.1) only.
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (5 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 30kD band observed in Human Kidney lysates (calculated MW of 30.9kD according to NP_000894.1). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NQO1
P15559 NM_000903 NP_000894.1

Validation

NQO1 Antibody - Anti-NQO1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-NQO1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-NQO1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

LSBio Ratings

IHC-plus™ NQO1 Antibody (aa134-146) for IHC, IF/Immunofluorescence, WB/Western LS-B8354 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/22/2024