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| Catalog Number | Size | Price |
|---|---|---|
| LS-B14132-50 | 50 µg (0.5 mg/ml) | $460 |
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IHC‑plus™ Polyclonal Goat anti‑Human PCNA Antibody (aa177‑189, IHC, WB) LS‑B14132
IHC‑plus™ Polyclonal Goat anti‑Human PCNA Antibody (aa177‑189, IHC, WB) LS‑B14132
Note: This antibody replaces LS-C186570
Antibody:
PCNA Goat anti-Human Polyclonal (aa177-189) Antibody
Application:
IHC, IHC-P, WB, ELISA, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
PCNA Goat anti-Human Polyclonal (aa177-189) Antibody
Application:
IHC, IHC-P, WB, ELISA, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Specifications
Description
PCNA antibody LS-B14132 is an unconjugated goat polyclonal antibody to PCNA (aa177-189) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Host
Goat
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-NGNIKLSQTSNVD, from the internal region of the protein sequence according to NP_002583.1.
Epitope
aa177-189
Specificity
Human PCNA / Cyclin. Reported variants represent identical protein: NP_872590.1, NP_002583.1
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.01 - 0.03 µg/ml)
- ELISA
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 30kD band observed in lysates of cell line HeLa (calculated MW of 28.8kD according to NP_002583.1). Approx. 33kD band was observed in lysates of cell line NIH3T3 and of Mouse and Rat Testis. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gilljam et al,, J Cell Biol. 2009 Sep 7;186(5):645-54.). Recommended concentration: 0.01-0.03 ug/ml. Enzyme immunoassay: This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with LS-C155050.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PCNA
Validation
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
IHC-plus™ PCNA Antibody (aa177-189) for IHC, WB/Western, ELISA LS-B14132 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Fish
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Rat, Human, Mouse, Chicken, Zebrafish, Drosophila, Yeast, Primate
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Rat, Chicken, Xenopus
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Rat, Human, Mouse, Cat, Dog, Ferret, Hamster, Horse, Rabbit, Sheep, Chicken, Xenopus, Atlantic salmon, Monkey-Cynomolgus, Monkey-Rhesus
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
