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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3003-50 | 50 µl (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human STEAP1 / STEAP Antibody (clone J2D2, aa1‑70, IHC, IF, WB) LS‑B3003
IHC‑plus™ Monoclonal Mouse anti‑Human STEAP1 / STEAP Antibody (clone J2D2, aa1‑70, IHC, IF, WB) LS‑B3003
Note: This antibody replaces LS-C93798, LS-C91675
Antibody:
STEAP1 / STEAP Mouse anti-Human Monoclonal (aa1-70) (J2D2) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
STEAP1 / STEAP Mouse anti-Human Monoclonal (aa1-70) (J2D2) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
STEAP antibody LS-B3003 is an unconjugated mouse monoclonal antibody to human STEAP (STEAP1) (aa1-70). Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human STEAP1 / STEAP
Synonyms
STEAP1 | Metalloreductase STEAP1 | PRSS24 | STEAP
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Clone
J2D2
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human STEAP1 (1-70aa) purified from E. coli. NCBI Accession No: NP_036581.
Epitope
aa1-70
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- ICC
- Immunofluorescence
- Western blot (1:500 - 1:1000)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3003 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3003 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
Storage
Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About STEAP1 / STEAP
Validation
Anti-STEAP1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-STEAP1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-STEAP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-STEAP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ STEAP1 / STEAP Antibody (aa1-70, clone J2D2) for IHC, ICC, IF/Immunofluorescence, WB/Western, ELISA LS-B3003 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human
Applications:
IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Rat
Applications:
IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
Date Requested: 12/21/2024
