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Catalog Number Size Price
LS-B428-50 50 µg (1.6 mg/ml) $515 
TG / Thyroglobulin Antibody - Anti-TG / Thyroglobulin antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TG / Thyroglobulin Antibody - Anti-Thyroglobulin Antibody - Western Blot. Western blot of Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kD band corresponding to human thyroglobulin (left lane 3 ug, right lane 3.0 ug) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] ( and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.
TG / Thyroglobulin Antibody - Anti-TG / Thyroglobulin antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TG / Thyroglobulin Antibody - Anti-Thyroglobulin Antibody - Western Blot. Western blot of Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kD band corresponding to human thyroglobulin (left lane 3 ug, right lane 3.0 ug) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] ( and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.
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IHC‑plus™ Monoclonal Mouse anti‑Human TG / Thyroglobulin Antibody (clone 2H11, IHC, WB) LS‑B428

IHC‑plus™ Monoclonal Mouse anti‑Human TG / Thyroglobulin Antibody (clone 2H11, IHC, WB) LS‑B428

Note: This antibody replaces LS-C18898
Antibody:
TG / Thyroglobulin Mouse anti-Human Monoclonal (2H11) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Human, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$515
LS-B428-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TG / Thyroglobulin Mouse anti-Human Monoclonal (2H11) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Human, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
Thyroglobulin antibody LS-B428 is an unconjugated mouse monoclonal antibody to Thyroglobulin (TG) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TG / Thyroglobulin
Synonyms
TG | AITD3 | TDH3 | Thyroglobulin | TGN
Host
Mouse
Reactivity
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
2H11
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with human thyroglobulin protein.
Specificity
Reacts specifically with thyroglobulin in human tissues. The antibody recognizes a 330-kDa band corresponding to thyroglobulin. Cross reactivity with thyroglobulin from mouse, rat and dog will occur. Cross reactivity with thyroglobulin from other sources has not been determined.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • IHC - Frozen (1:50 - 1:200)
  • Western blot (1:50 - 1:200)
  • ELISA (1:20000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B428 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B428 was determined to be 5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TG / Thyroglobulin
P01266 NM_003235 NP_003226.4

Validation

TG / Thyroglobulin Antibody - Anti-TG / Thyroglobulin antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TG / Thyroglobulin antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TG / Thyroglobulin antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ TG / Thyroglobulin Antibody (clone 2H11) for IHC, WB/Western, ELISA LS-B428 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024