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Catalog Number Size Price
LS-B2248-50 50 µg (1 mg/ml) $460 
TLR8 Antibody - Anti-TLR8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TLR8 Antibody - Western blot of TLR8 in cell lysates from 293 transfected with human TLR8 using antibody at a concentration of 2 ug/ml.
TLR8 Antibody - Intracellular flow analysis of TLR8 in1x10^6 Ramos cells using 0.5 ug of antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; purple represents anti-TLR8 antibody.
TLR8 Antibody - Anti-TLR8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TLR8 Antibody - Western blot of TLR8 in cell lysates from 293 transfected with human TLR8 using antibody at a concentration of 2 ug/ml.
TLR8 Antibody - Intracellular flow analysis of TLR8 in1x10^6 Ramos cells using 0.5 ug of antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; purple represents anti-TLR8 antibody.
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IHC‑plus™ Monoclonal Mouse anti‑Human TLR8 Antibody (clone 44C143, aa750‑850, IHC, WB) LS‑B2248

IHC‑plus™ Monoclonal Mouse anti‑Human TLR8 Antibody (clone 44C143, aa750‑850, IHC, WB) LS‑B2248

Note: This antibody replaces LS-C549, LS-C10769
Antibody:
TLR8 Mouse anti-Human Monoclonal (aa750-850) (44C143) Antibody
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B2248-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TLR8 Mouse anti-Human Monoclonal (aa750-850) (44C143) Antibody
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
TLR8 antibody LS-B2248 is an unconjugated mouse monoclonal antibody to TLR8 (aa750-850) from human. It is reactive with human and mouse. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TLR8
Synonyms
TLR8 | CD288 | Toll-like receptor 8 | CD288 antigen
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
44C143
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human TLR8.
Epitope
aa750-850
Specificity
A KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850. It will cross-react with mouse TLR8.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2248 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2248 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.4, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TLR8
Q9NR97 NM_138636 NP_619542.1

Validation

TLR8 Antibody - Anti-TLR8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ TLR8 Antibody (aa750-850, clone 44C143) for IHC, WB/Western, Flow LS-B2248 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/22/2024