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Catalog Number	Size	Price
LS-B16084-50	50 µl (1 mg/ml)	$460

TOP2A / Topoisomerase II Alpha Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

TOP2A / Topoisomerase II Alpha Antibody - Immunohistochemical analysis of Topoisomerase 2 alpha staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

TOP2A / Topoisomerase II Alpha Antibody - Immunofluorescent analysis of Topoisomerase 2 alpha staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

TOP2A / Topoisomerase II Alpha Antibody - Western blot analysis of Topoisomerase 2 alpha expression in MDAMB468 (A); MDAMB231 (B); HeLa (C) whole cell lysates.

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IHC‑plus™ Polyclonal Rabbit anti‑Human TOP2A / Topoisomerase II Alpha Antibody (N‑Terminus, IHC, IF, WB) LS‑B16084

Name: IHC-plus™ TOP2A / Topoisomerase II Alpha Polyclonal Antibody | LSBio
Brand: Lifespan BioSciences
SKU: LS-B16084-50
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C352956

Antibody:

TOP2A / Topoisomerase II Alpha Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human

Format:

Unconjugated, Unmodified

$460

LS-B16084-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

TOP2A / Topoisomerase II Alpha Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Topoisomerase II Alpha antibody LS-B16084 is an unconjugated rabbit polyclonal antibody to human Topoisomerase II Alpha (TOP2A) (N-Terminus). Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human TOP2A / Topoisomerase II Alpha

Synonyms

See All TOP2A / Topoisomerase II Alpha Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human Topoisomerase 2 alpha.

Epitope

N-Terminus

Specificity

Recognizes endogenous levels of Topoisomerase 2 alpha protein.

Applications

IHC
IHC - Paraffin (1:50)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)
Immunoprecipitation (1:10 - 1:100)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About TOP2A / Topoisomerase II Alpha

Uniprot: P11388 NCBI: NM_001067 NP_001058.2