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Catalog Number	Size	Price
LS-B9320-200	200 µl	$460

UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

UCHL1 / PGP9.5 Antibody - Fluorescent image of U251 cell stained with UCHL1 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UCHL1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). UCHL1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

UCHL1 / PGP9.5 Antibody - UCHL1 Antibody western blot of U266,NCI-H1299,mouse Neuro-2a cell line and mouse brain tissue lysates (35 ug/lane). The UCHL1 antibody detected the UCHL1 protein (arrow).

UCHL1 / PGP9.5 Antibody - Western blot of lysates from SH-SY5Y cell line and mouse brain tissue (from left to right),using UCHL1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

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IHC‑plus™ Polyclonal Rabbit anti‑Human UCHL1 / PGP9.5 Antibody (aa187‑214, IHC, IF, WB) LS‑B9320

Name: IHC-plus™ UCHL1 / PGP9.5 Polyclonal Antibody Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B9320-200
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C160410

Antibody:

UCHL1 / PGP9.5 Rabbit anti-Human Polyclonal (aa187-214) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$460

LS-B9320-200

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

UCHL1 / PGP9.5 Rabbit anti-Human Polyclonal (aa187-214) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

PGP9.5 antibody LS-B9320 is an unconjugated rabbit polyclonal antibody to PGP9.5 (UCHL1) (aa187-214) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human UCHL1 / PGP9.5

Synonyms

See All UCHL1 / PGP9.5 Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Predicted

Monkey, Mouse, Pig, Cow (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Epitope

aa187-214

Specificity

This UCHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-214 amino acids from the C-terminal region of human UCHL1.

Applications

IHC
IHC - Paraffin (5 µg/ml)
Immunofluorescence (1:10 - 1:50)
Western blot (1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About UCHL1 / PGP9.5

Uniprot: P09936 NCBI: NM_004181 NP_004172.2

Validation

Anti-UCHL1 / PGP9.5 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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About LSBio Validation process