Human IL13

Rat

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Purified

Azide-free

COS-expressed recombinant human IL-13.

Recognizes human Interleukin-13 (IL-13).

• Immunofluorescence
• Western blot
• Flow Cytometry
• ELISA
• Neutralization
• Sandwich ELISA

ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying human IL-13 protein levels.1, 2 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-13-positive cells within mixed cell populations. Recombinant human IL-13, or Rat IgG1 should be used as a specificity control. Western Blot: Useful for Western blot applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-13 under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing human IL-13 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS, No preservatives added

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P35225 NCBI: NM_002188 NP_002179.2